Canine parvovirus gene grow in E.Coli using molasses medium which can be use as a vaccine.

The Canine parvovirus gene is inserted in E.coli (DH5α) strain. Grow the recombinant E. coli for the plasmid DNA using molasses medium to make the vaccine. During the initial studies I have found that the molasses can manipulate the C/N ratio as per requirement of cell. Molasses is one of the best alternatives as it is cheap and can be easily manipulated. During the experiment the O.D of culture and O.D of plasmid DNA was observed in respective of different optimization method. I observed that the O.D value has increase to i.e. 2.129, as it was earlier i.e. 0.393. The quality and quantity of the plasmid DNA was very good. It is possible to produce vaccine by molasses medium. The paper is opening a new face of study.